Composite

Part:BBa_K4235010:Design

Designed by: Maulik Masaliya   Group: iGEM22_Stony_Brook   (2022-09-12)


mini-attTn7 segment: Tn7R+GmR circuit+Polyhedrin+PROS1+SV40+Tn7L


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2914
    Illegal BglII site found at 37
    Illegal BglII site found at 568
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2914
    Illegal XbaI site found at 2227
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The miniatt-Tn7 transposon segment is part of the vector pFastBac BBa_K4235002

References

Bac-to-bac baculovirus expression systems . (n.d.). Retrieved September 22, 2022. http://wolfson.huji.ac.il/expression/bac.pdf